Journal: Scientific Reports

Article Title: Differential epigenetic regulation between the alternative promoters, PRDM1α and PRDM1β , of the tumour suppressor gene PRDM1 in human multiple myeloma cells

doi: 10.1038/s41598-020-72946-z

Figure Lengend Snippet: Schematic representation of the regulation of human PRDM1 promoters in multiple myeloma. a Factors such as SP1, SP3 and EGR1 act on the PRDM1α promoter, inducing its transcription , the opposite effect as that of PAX5 . On the PRDM1β promoter, TP53 (as an activator) , and FOX factors (as inhibitors) can regulate PRDM1β transcription. Regarding epigenetic regulation, methylation seems to play a decisive role in PRDM1β expression but not in PRDM1α expression. In the case of histone modifications, H3K4me1 and H3K9me2 marks seem to be linked to the repression of the PRDM1β promoter. Activators and inhibitors are represented above and under the DNA helix, respectively. Filled and open lollipops represent the methylation and demethylation status of the promoter, respectively. This figure was created using Corel Draw 2018 version 20.1.0.708 software (Corel Corporation, Ottawa, Canada). b Schematic representation showing how PRDM1β expression tilts the balance in favour of proliferation due to a diminished apoptosis rate. Inhibiting PRDM1β expression using drugs targeting epigenetic elements at its promoter may restore the balance to maintain normal PC cell fate.

Article Snippet: This figure was created using Corel Draw 2018 version 20.1.0.708 software (Corel Corporation, Ottawa, Canada). b Schematic representation showing how PRDM1β expression tilts the balance in favour of proliferation due to a diminished apoptosis rate.

Techniques: Methylation, Expressing, Software